HER-2/neu belongs to the EGFR family of thyrosine  kinase  receptors. Its overexpression  characterizes about 25% of patients with breast cancers candidate to treatment with  anti HER-2 targeting therapies.

PRODUCT

1. Each vial contains the indicated amount of IgG (caprylic acid purified) in 0.1% gelatine and  0.05% NaN3.

2. Unpurified reagent is provided at the indicated amounts with 0.1% NaN3.

Centrifuge the vial prior to use.

SPECIFICITY

The murine monoclonal antibody MP-AA-2 (IgG1) has been generated using NHI-3T3 cells transfected with the human receptor (ref.1)  It recognizes an epitope of the extracellular domain of the human  gp185HER-2/neu  resistant to formalin fixation. It recognizes the receptor with an affinity of 2x109M-1. The reagent does not crossreact with non human receptors (see references).

STORAGE

Store at 4° C, avoid repeated freezing-thawing. Stable for one year.

SHIPPING CONDITIONS

Room temperature.

RESEARCH USE

This antibody is for laboratory research use only, not for human or in-vivo use.

APPLICATIONS

Flow cytometry, 5-10 µg/ml

Immunohistochemistry, (fluorescence, enzymatic), On acetone fixed cryostat sections and cytospins (5-10 µg/ml); paraffin embedded tissue sections (enzymatic 20 µg/ml) with no need of antigen retrieving procedures. MP-AA-2 and FISH analysis have been shown over  80% concordance (ref.4)

Immunoprecipitation, 2 µg, using rabbit anti-mouse Ig and protein ASepharose beads (ref.1).

REFERENCES
1. Hybridoma (1992) 11: 519
2. J Natl Cancer Inst (1997) 89:318
3. J Immunother (2001) 24:221
4. Cancer Genet Cytogen (2002) 33:66
5. J Cellular Physiol (2005) 204:106